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Tiffany Barszczewski

Research Associate III

teams /
Allenite

Tiffany joined the Allen Institute for Cell Science in 2023 as a Research Associate III. She specializes in the maintenance and differentiation of human induced pluripotent stem (hiPS) cell lines with endogenous fluorescent tags. She uses a diverse array of techniques to prepare these cells for downstream assays, which are critical for elucidating cellular organization, behavior, and identity.

Prior to her current role, Tiffany earned her B.S. (Hons) with Distinction in Biomedical Physiology from Simon Fraser University. In her graduate-level work in the Tibbits Lab at the Cellular and Regenerative Medicine Centre (BC Children’s Hospital Research Institute), her research focused on using CRISPR gene editing to investigate inherited arrhythmias and cardiomyopathies. She developed and optimized image-based assays to measure changes in contractility and morphology in edited hiPS cell-derived cardiomyocytes.

Research focus: Tiffany’s work is focused within several key areas, including: hiPS cell-derived endothelial cells under various shear stresses, the transition from 2D flat colonies to 3D lumenoids, hiPS cell differentiation into various cell lineages. Her technical expertise spans cell line maintenance, drug and small molecule assays, targeted CRISPRi, immunofluorescence, ddPCR, and live-cell microscopy. She is passionate about applying her skills to advance our understanding of cell biology and is particularly interested in the development of new technologies for high-throughput cellular analysis.

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